By Wayne W Umbreit
Meant for researchers in utilized microbiology and environmental engineers, this ebook covers such themes as environmental review of biotechnological procedures and microbial variations of haloaromatic and haloaliphatic compounds.
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Extra info for Advances in Applied Microbiology, Vol. 3
The inherent disadvantage of bulkiness and the time-consuming process of regenerating the desiccant have made the Cryochem apparatus virtually obsolete. The advent of mechanical refrigeration has offset the former advantage of the Cryochem apparatus, that of not requiring dry ice. FIG. 3. A simplified but effective lyophilization apparatus, Naval Biological Laboratory. , Gardiner, New York, makes a number of laboratory scale lyophilization instruments which have considerable utility. Figure 4 shows a drying unit with a small condenser of the “pig” type on the upper shelf of the cart and a secondary condenser below it.
Murcescens survived lyophilization about as well in spent growth medium as in any of the other materials tested. From the practical point of view additional steps, such as centrifugation, are to be avoided. Furthermore it seems likely that the presence of at least some spent medium, if it is not too acid or alkaline, is desirable for the preservation of all organisms, Although it has been generally believed that death caused by freezing is a result of puncture of the cell wall by ice crystal formation, there is little evidence that this is actually the cause of death.
37°C. 5°C. 37°C. 6 Months 6 Months 6 Months 6 Months 10% Sucrose and 1% gelatin - 0 Mycobacterium tuberculosis (BCG) 50% Glucose Milk whey plus 3 % glucose 50 % Glucose 1% Sucrose 1 % Sodium glutamate 25°C. 6 Months 12 Months l'er cent viability" P"4OC. 2"-4"C. 2'4°C. 2"-4"C. 5 Retained immunogenicity Decreased immunogenicity 40 Cho and Obayashi (1956) and Obayashi and Cho (1957) Cho et al. (1956) Kurylowicz el al. (1958) 37 PRESERVATION OF BACTERIA BY LYOPHILIZATION TABLE I (Continued) Storage Organism and suspending fluid Temperature Time 50% Glucose 18 Months Retained immunogenicity 4°C.
Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit