By Saul L. Neidleman (ed.), Allen I. Laskin (ed.)

ISBN-10: 012002635X

ISBN-13: 9780120026357

Meant for researchers in utilized microbiology and environmental engineers, this e-book covers such issues as environmental overview of biotechnological tactics and microbial ameliorations of haloaromatic and haloaliphatic compounds.

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Additional info for Advances in Applied Microbiology, Vol. 35

Sample text

Minagawa and Hamaishi (1962) claimed the isolation of an improved mutant by subjecting B. subtilis to prolonged X-ray treatment. 5°/0 NaCl and 3% agar, was then regrown on solid medium for 1 2 hours at 33°C. It was then subjected to X-ray treatment using an X-ray intensity of 24-50 roentgens, obtained by placing the generator -30 cm away from the colony. The duration of exposure to the X radiation was at least 30 minutes in a range of 1-4 hours. The colony was then cultured in plane culture in a shallow tray at 33°C for 24 hours using solid medium of the same composition.

Megaterium 16M, while the enzyme 36 B. K. LONSANE AND M. V. 0). The production of such a high titer of enzyme in the SSF process is of significant commercial importance in that the authors stressed that it was unattainable in SmF. The coproduction of proteases along with a-amylase is a general characteristic of Bacillus strains in SmF. A similar characteristic was also reported in the SSF process, and it was even stressed that the preferential production of either of these enzymes can be achieved by using appropriate solid substrate or cultural parameters (Minagawa and Hamaishi, 1962).

It is evident that the ratio used varies with the bacterial culture used in the process. 5 Reference Ramesh and Lonsane (1987a) Qadeer et al. (1980); Lulla and Subrahmanyan (1954); Ramesh and Lonsane (1987b) Beckord et al. , Ltd. (1961) Arima (1964) Feniksova et al. 5 was optimum and nearly doubles enzyme titers as compared to a ratio of 1 : 2. Buffer-WB ratios ranging from 1 to 3 were also studied by Ramesh and Lonsane (1987a), and the ratio of 2 : 1 was found to be optimum. Further increase in the ratio resulted in higher enzyme production, but the increase was not significant.

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Advances in Applied Microbiology, Vol. 35 by Saul L. Neidleman (ed.), Allen I. Laskin (ed.)


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